Let-7a对犬流感病毒诱导细胞凋亡调控作用的初步研究

doi:10.11843/j.issn.0366-6964.2016.06.021

Abstract

Abstract:

Canine influenza (CI) is an infectious disease caused by canine influenza virus (CIV) infection，which is mainly manifested as acute respiratory symptoms.In order to explore the effect of let-7a on CIV induced cells apoptosis，MDCK cells were infected by CIV in vitro，and AO/EB staining and flow cytometry were used to detect cells apoptosis，and the transcription of let-7a was also determined by real time PCR，and the effect of let-7a on cell apoptosis was evaluated by the transfection of let-7a.The results showed that CIV could induce apoptosis of MDCK cells and downregulate the transcription of let-7a in infected cells，and over-transcription of let-7a could effectively inhibit the virus induced apoptosis.This study showe that let-7a plays an anti-apoptotic role in CIV infected cells，and the low transcription of let-7a promotes apoptosis，and this mechanism may play a role in the respiratory tract injury of CIV infected dogs.

CLC Number:

S855.3

HU Ren-jun，TU Li-qing，SUN Ling-shuang，JIA Kun，YUAN Li-guo，LI Shou-jun. Study on Regulation Function of Let-7a on Canine Influenza Virus Induced Cells Apoptosis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(6): 1247-1252.

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References

［1］PAYUNGPORN S，CRAWFORD P C，KOUO T S，et al.Influenza A virus (H3N8) in dogs with respiratory disease，Florida ［J］.Emerg Infect Dis，2008，14(6)：902-908.
［2］SONG D，KANG B，LEE C，et al.Transmission of avian influenza virus (H3N2) to dogs［J］.Emerg Infect Dis，2008，14(5)：741-746.
［3］LI S J，SHI Z H，JIAO P R，et al.Avian-origin H3N2 canine influenza A viruses in Southern China［J］.Infect Genet Evol，2010，10(8)：1286-1288.
［4］PARK S J，MOON H J，KANG B K，et al.Complete genome sequence of an avian-origin H3N2 canine influenza virus isolated from dogs in South Korea［J］.J Virol，2012，86(17)：9548-9549.
［5］BUTLER D.Thai dogs carry bird-flu virus，but will they spread it?［J］.Nature，2006，439(7078)：773.
［6］CHEN Y，MO Y N，ZHOU H B，et al.Emergence of human-like H3N2 influenza viruses in pet dogs in Guangxi，China［J］.Virol J，2015，12：10.
［7］THOMSON J M，NEWMAN M，PARKER J S，et al.Extensive post-transcriptional regulation of microRNAs and its implications for cancer［J］.Genes Dev，2006，20(16)：2202-2207.
［8］SKALSKY R L，CULLEN B R.Viruses，microRNAs，and host interactions ［J］.Annu Rev Microbiol，2010，64：123-141.
［9］ZHAO F R，SU S，ZHOU D H，et al.Comparative analysis of microRNAs from the lungs and trachea of dogs (Canis familiaris) infected with canine influenza virus［J］.Infect Genet Evol，2014，21：367-374.
［10］KANG Y M，KIM H M，KU K B，et al.H3N2 canine influenza virus causes severe morbidity in dogs with induction of genes related to inflammation and apoptosis［J］.Vet Res，2013，44：92.
［11］SU S，TIAN J，HONG M L，et al.Global and quantitative proteomic analysis of dogs infected by avian-like H3N2 canine influenza virus［J］.Front Microbiol，2015，6：228.
［12］CHEN Y，MO Y N，ZHOU H B，et al.Emergence of human-like H3N2 influenza viruses in pet dogs in Guangxi，China［J］.Virol J，2015，12：10.
［13］HONG M，NA W，YEOM M，et al.Complete genome sequences of H3N2 canine influenza virus with the matrix gene from the pandemic A/H1N1 virus ［J］.Genome Announc，2014，2(5)：e01010-e01014.
［14］王存连，魏东，徐明举，等.ｐ３８ＭＡＰＫ信号通路在猪源Ｈ９Ｎ２流感病毒导致小鼠肺损伤中作用的研究［J］.畜牧兽医学报，2014，45(2)：281-288.
WANG C L，WEI D，XU M J，et al.The role of p38MAPK in acute lung injury induced by H9N2 influenza virus isolated from swine［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(2)：281-288.(in Chinese)
［15］李玉谷，叶远兰，崔聪颖，等.1株H5N1亚型高致病力禽流感病毒人工感染鸭的细胞凋亡观察［J］.畜牧兽医学报，2009，40(7)：1069-1073.
LI Y G，YE Y L，CUI C Y，et al.Induction of apoptosis by a highly pathogenic avian influenza virus，A/duck/Guangdong/220/ 2004(H5N1) in experimentally infected ducks［J］.Acta Veterinaria et Zootechnica Sinica，2009，40(7)：1069-1073.(in Chinese)
［16］郑良焰，陈龙，徐家华，等.Ｈ９亚型禽流感病毒感染引起ＭＤＣＫ细胞内抗氧化功能的变化［J］.中国畜牧兽医，2013，40(11)：175-179.
ZHENG L Y，CHEN L，XU J H，et al.Effect of avian influenza virus subtype H9 on antioxidant function in MDCK cells［J］.China Animal Husbandry and Veterinary Medicine，2013，40(11)：175-179.(in Chinese)
［17］BARTEL D P.MicroRNAs：Genomics，biogenesis，mechanism，and function ［J］.Cell，2004，116(2)：281-297.
［18］BAO M H，ZHANG Y W，LOU X Y，et al.Protective effects of let-7a and let-7b on oxidized low-density lipoprotein induced endothelial cell injuries ［J］.PLoS One，2014，9(9)：e106540.
［19］HAM O，LEE S Y，LEE C Y，et al.Let-7b suppresses apoptosis and autophagy of human mesenchymal stem cells transplanted into ischemia/reperfusion injured heart 7by targeting caspase-3 ［J］.Stem Cell Res Ther，2015，6：147.

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Study on Regulation Function of Let-7a on Canine Influenza Virus Induced Cells Apoptosis

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